Molecular diagnostic reagent

Folate three metabolizability test kit

Folate three metabolizability test kit

The kit (PCR fluorescence probe method) developed by Yingsheng biology is used for qualitative detection of folate...

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The PCR fluorescence probe kit developed by Yingsheng biology is used to detect the base mutation of folate metabolism related genes (including MTHFR gene C677T, A1298C, MTRR gene A66G) in human peripheral blood. It is suitable for the assistant diagnosis of folate metabolism ability in clinical patients. Through the qualitative detection of genotypes of MTHFR gene C677T, A1298C and MTRR gene A66G, it can provide reference for clinicians to evaluate the risk degree of folate metabolism ability disorder.

[Significance of detection]
Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in the methionine folate metabolism system. Together with MTRR, MTHFR maintains the normal metabolism of folate. In addition, it is also involved in maintaining the normal level of homocysteine in the body. MTHFR and MTRR Gene mutation can reduce the activity of the key enzyme of folate metabolism, cause folate metabolism disorder, folate deficiency or utilization ability defect, cause the risk of neonatal neural tube defect, Down's syndrome, cleft lip and palate, pregnancy hypertension or spontaneous abortion and other diseases significantly increased, the childbearing age and pregnant women with folate metabolism risk need to supplement more Folic acid can achieve the desired effect. However, excessive supplement of folic acid will lead to zinc deficiency in the body, slow growth of the fetus during pregnancy and low birth weight; it will also cover up anemia and neurological disorders caused by vitamin B12 deficiency, and reduce the number of natural killer cells in the body and immunity of the body. This kit is suitable for the auxiliary diagnosis of folate metabolism ability of clinical patients. Through the detection of C677T, A1298C of MTHFR gene and A66G of MTRR gene, the folate metabolism ability is evaluated, which provides scientific guidance for the individualized folate supplement scheme of clinical patients.

Fluorescence PCR method

32 per person / box

[Product features]
This kit uses TaqMan probe labeled fluorescent PCR technology to design highly specific primers and probes for target genes. It has the characteristics of 1, convenient and fast, PCR amplification within 2 hours can directly produce results, 2, small investment, the existing dual channel PCR instrument can complete the experiment, no additional equipment, 3, no pollution, closed detection in the whole process, prevention of cross contamination, 4, accurate results, 100% consistent with the sequencing results, and elimination of false negative and false positive results.