[Significance of detection]
HLA-B27 gene is an allele on the B site of the major histocompatibility complex in human, which has a high correlation with ankylosing spondylitis. More than 90% of patients with ankylosing spondylitis have positive HLA-B27 antigen expression, while the positive rate in the general population is only 5% - 10%. Ankylosing spondylitis is a chronic inflammatory disease. The incidence rate in China is about 3%, which mainly invades the spine, the adjacent tissues and the lateral joints. It can also be accompanied by extra articular manifestations. Severe cases can cause spinal deformity and ankylosis. Therefore, the detection of HLA-B27 gene level is of great significance in the diagnosis of ankylosing spondylitis.
Fluorescence PCR method
48 per person / box
This kit uses TaqMan probe labeled fluorescent PCR technology to design highly specific primers and probes for target genes. It has the characteristics of 1, convenient and fast, PCR amplification within 2 hours can directly produce results, 2, small investment, the existing dual channel PCR instrument can complete the experiment, no additional equipment, 3, no pollution, closed detection in the whole process, prevention of cross contamination, 4, accurate results, 100% consistent with the sequencing results, and elimination of false negative and false positive results.